Recombinant Human Granulocyte Colony-Stimulating Factor/G-CSF (C002)

目录 规格 价格
【G-CSF产品说明】:
Recombinant Human Granulocyte Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Thr31-Pro204 is expressed.
【G-CSF质量控制】:
Measured by the dose-dependent proliferation of murine NFS-60 indicator cells.
ED50 is less than 0.1 ng/ml. Specific Activity of 6.0 x 10^7 IU/ mg.
Greater than 95% as determined by reducing SDS-PAGE.
【G-CSF制剂】:
Lyophilized
【G-CSF保存】:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
【G-CSF复溶】:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
【关于G-CSF】:
Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.
参考文献

  • Welte T, Kim I S, Tian L, et al.
    Oncogenic mTOR signalling recruits myeloid-derived suppressor cells to promote tumour initiation.
    Nature Cell Biology(2016)

  • Kryndushkin D, Rao V A.
    Comparative Effects of Metal-Catalyzed Oxidizing Systems on Carbonylation and Integrity of Therapeutic Proteins.
    Pharmaceutical research(2015)
For research use ONLY.