Recombinant Human GM-CSF/CSF2 (E.coli) (C003)

目录 规格 价格
【GM-CSF产品说明】:
Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Glu144 is expressed.
【GM-CSF质量控制】:
Measured by the dose-dependent stimulation of human TF-1 cell proliferation.
ED50 is less than 0.1 ng/ml. Specific Activity of 1.0 x 10^7 IU/ mg.
Greater than 95% as determined by reducing SDS-PAGE.
【GM-CSF制剂】:
Lyophilized
【GM-CSF保存】:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
【GM-CSF复溶】:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
【关于GM-CSF】:
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
参考文献

  • Sullivan C J, Pendleton E D, Sasmor H H.
    A cell‐free expression and purification process for rapid production of protein biologics.
    Biotechnology journal(2015)

  • Li Z, Cao G, Xue R, et al..
    Methods for transferring gene into silkworm and expression of hGM-CSF in silk glands of transgenic silkworm.
    Journal of Chemical and Pharmaceutical Research(2014)
For research use ONLY.