Recombinant Human Insulin-Like Growth Factor I/IGF-1(4-70) (RG) (C031)

目录 规格 价格
【IGF-I产品说明】:
Recombinant Human Insulin-like Growth Factor I(4-70) is produced by our E.coli expression system and the target gene encoding Thr52-Ala118 is expressed.
【IGF-I质量控制】:
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells
The ED50 for this effect is typically 8 ng/mL.
Greater than 95% as determined by reducing SDS-PAGE.
【IGF-I制剂】:
Lyophilized
【IGF-I保存】:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
【IGF-I复溶】:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
【关于IGF-I】:
Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids with 94% identity with mouse IGF1 and exhibits cross-species activity. IGF1 binds IGF-1R, IGF-2R, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF1 expression is regulated by growth hormone.
参考文献

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    A serine protease extracted from Trichosanthes kirilowii induces apoptosis via the PI3K/AKT-mediated mitochondrial pathway in human colorectal adenocarcinoma cells.
    Food & function(2016)

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    Furazolidone induces apoptosis through activating reactive oxygen species-dependent mitochondrial signaling pathway and suppressing PI3K/Akt signaling pathway in HepG2 cells[J]..
    Food and Chemical Toxicology, 2015, 75: 173-186.(2015)

  • Zhou Y, Zhang S, Deng S.
    ML-7 amplifies the quinocetone-induced cell death through akt and MAPK-mediated apoptosis on HepG2 cell line.
    Toxicology mechanisms and methods(2015)
For research use ONLY.