Recombinant Human β-Nerve Growth Factor/β-NGF (Glu19-Ala241) (C078)

目录 规格 价格
【NGF(with pro peptide)产品说明】:
Recombinant Human pro-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Glu19-Ala241 is expressed.
【NGF(with pro peptide)质量控制】:
Greater than 95% as determined by reducing SDS-PAGE.
【NGF(with pro peptide)制剂】:
Lyophilized
【NGF(with pro peptide)保存】:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
【NGF(with pro peptide)复溶】:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
【关于NGF(with pro peptide)】:
The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.
参考文献

  • Duan L, Chen B Y, Sun X L, et al..
    LPS-Induced proNGF Synthesis and Release in the N9 and BV2 Microglial Cells: A New Pathway Underling Microglial Toxicity in Neuroinflammation.
    PLoS ONE(2013)

  • Pontus Forsell, Helena Almqvist, Per Hillertz, Tomas Åkerud, Magdalena Otrocka, Lina Eisele, Kai Sun, Henrik Andersson, Shephali Trivedi, Anna Ridderstad Wollberg, Niek Dekker, Didier Rottici, and Kristian Sandberg .
    The Use of TrkA-PathHunter Assay in High-Throughput Screening to Identify Compounds That Affect Nerve Growth Factor Signaling.
    Journal of Biomolecular Screening(2013)

  • Jiao-Ning Shen, Deng-Shun Wang, Rui Wang.
    The protection of acetylcholinesterase inhibitor on β-amyloid-induced the injury of neurite outgrowth via regulating axon guidance related genes expression in neuronal cells..
    Int J Clin Exp Pathol(2012)
For research use ONLY.