Recombinant Human Interleukin-10/IL-10 (N-6His) (C027)

目录 规格 价格
Recombinant Human Interleukin-10 is produced by our E.coli expression system and the target gene encoding Ser19-Asn178 is expressed with a 6His tag at the N-terminus.
Measured by the dose-dependent co-stimulation with murine IL-4 of MC-9 cells.
ED50 is less than 2 ng/ml. Specific Activity of 1.5 x 10^6 IU/mg.
Greater than 95% as determined by reducing SDS-PAGE.
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Interleukin 10(IL10), also known as cytokine synthesis inhibitory factor (CSIF),is a secreted protein and belongs to the IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . IL-10 is an anti-inflammatory TH2 cytokine that has a critical role in limiting the immune response to pathogens to prevent host damage. As IL-10 in produced in several T helper populations, it is proposed that it provides a feedback loop to limit the effector functions of macrophages and DCs on T cells. Once expressed, IL-10 signals through the IL-10 receptor (IL-10R) to activate STAT3. As IL-10 is a strong inhibitor of inflammation, it has become a viable biomarker for various diseases and conditions as well as a therapeutic molecule for certain conditions. In addition to elevated levels in parasitic infection, high expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations

  • Ruijin Zhang Haixia Zhang Xianbing Liu Qiang Fu Xiaoyan Xu Xuemei Hu.
    The immunoprotective role of interleukin-10 in abnormal pregnancy outcome induced by Toxoplasma gondii infection..
    Gynecol Obstet Invest(2011)
For research use ONLY.