Recombinant Human Interleukin-6/IL-6 (C009)

目录 规格 价格
Recombinant Human Interleukin-6 is produced by our E.coli expression system and the target gene encoding Pro29-Met212 is expressed.
Measured by the dose-dependent stimulation of human TF-1 cells.
ED50 is less than 0.1 ng/ml. Specific Activity of 5.0 x 10^7 IU/mg.
Greater than 95% as determined by reducing SDS-PAGE.
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.

  • Wang C, Hu L, Zhao L, et al..
    Inflammatory stress increases hepatic CD36 translational efficiency via activation of the mTOR signalling pathway[J]..
    PloS one(2014)

  • Mao M, Lei H, Liu Q, et al..
    Effects of miR-33a-5P on ABCA1/G1-Mediated Cholesterol Efflux under Inflammatory Stress in THP-1 Macrophages[J]..
    PloS one(2014)

  • Zhou C, Lei H, Chen Y, et al.
    Enhanced SCAP Glycosylation by Inflammation Induces Macrophage Foam Cell Formation.
    PloS one(2013)

  • Zhang N, Lei J Y, Lei H, et al.
    MicroRNA-101 overexpression by IL-6 and TNF-α Inhibits cholesterol efflux by suppressing ATP-binding cassette transporter A1 expression.
    Experimental Cell Research(2015)

  • Lee S, Lee M, Kim J B, et al.
    17β-estradiol exerts anticancer effects in anoikis-resistant hepatocellular carcinoma cell lines by targeting IL-6/STAT3 signaling.
    Biochemical and Biophysical Research Communications(2016)

  • Han K, Xu X, Chen G, et al..
    Identification of a promising PI3K inhibitor for the treatment of multiple myeloma through the structural optimization.
    Journal of hematology & oncology(2014)
For research use ONLY.