Recombinant Human Leukemia Inhibitory Factor/LIF (C017)

目录 规格 价格
【LIF/Leukemia inhibitory factor产品说明】:
Recombinant Human Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser23-Phe202 is expressed.
【LIF/Leukemia inhibitory factor质量控制】:
Measured by the M1 cell differentiation assay.
ED50 is less than 0.01 ng/ml. Specific Activity of 1.0 x 10^8 IU/mg.
Greater than 95% as determined by reducing SDS-PAGE.
【LIF/Leukemia inhibitory factor制剂】:
Lyophilized
【LIF/Leukemia inhibitory factor保存】:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
【LIF/Leukemia inhibitory factor复溶】:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
【关于LIF/Leukemia inhibitory factor】:
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
参考文献

  • Wu Y, Shen Y, Kang K, et al.
    Effects of estrogen on growth and smooth muscle differentiation of vascular wall-resident CD34+ stem/progenitor cells.
    Atherosclerosis(2015)

  • Zeng L, Qiu L, Yang X, et al.
    Isolation of lung multipotent stem cells using a novel microfluidic magnetic activated cell sorting system.
    Cell biology international(2015)

  • Shuling Pan, Li Li , Zhiyong Xu, Jiao Zhao.
    Effect of leukemia inhibitory factor on corneal nerve regeneration of rabbit eyes after laser in situ keratomileusis.
    Neuroscience Letters(2011)

  • 赵振国,罗福康,安江宏,段玉忠,孙建国,朱 波,陈正堂.
    AC133和EpCAM在人肺腺癌中的表达及双阳性细胞的分选.
    第二军医大学学报(2009)
For research use ONLY.