Recombinant Mouse Tumor Necrosis Factor/TNFα (CF09)

目录 规格 价格
【TNF-alpha产品说明】:
Recombinant Mouse Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed.
【TNF-alpha质量控制】:
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 6.8 pg/mL.
Greater than 95% as determined by reducing SDS-PAGE.
【TNF-alpha制剂】:
Lyophilized
【TNF-alpha保存】:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
【TNF-alpha复溶】:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
【关于TNF-alpha】:
Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
参考文献

  • Zhao L, Zhong S, Qu H, et al.
    Chronic inflammation aggravates metabolic disorders of hepatic fatty acids in high-fat diet-induced obese mice.
    Scientific reports(2015)

  • Liu Y, Zhu L, Liang S, et al.
    Galactose protects hepatocytes against TNF-α-induced apoptosis by promoting activation of the NF-κB signaling pathway in acute liver failure.
    Laboratory Investigation(2015)

  • Zhou C, Lei H, Chen Y, et al.
    Enhanced SCAP Glycosylation by Inflammation Induces Macrophage Foam Cell Formation.
    PloS one(2013)

  • Zhang N, Lei J Y, Lei H, et al.
    MicroRNA-101 overexpression by IL-6 and TNF-α Inhibits cholesterol efflux by suppressing ATP-binding cassette transporter A1 expression.
    Experimental Cell Research(2015)

  • Wu Y, Sun H, Song F, et al..
    DDIT3 overexpression increases odontoblastic potential of human dental pulp cells.
    Cell proliferation(2014)

  • Yu-Sheng Chen Hong-Ru Li Ming Lin Gang Chen Bao-Song Xie Neng-Luan Xu Li-Fang Lin .
    Livin abrogates apoptosis of SPC-A1 cell by regulating JNKI signaling pathway..
    Mol Biol Rep (2010)

  • Lv Q, Zhen Q, Liu L, et al.
    AMP-kinase pathway is involved in tumor necrosis factor alpha-induced lipid accumulation in human hepatoma cells.
    Life sciences(2015)
For research use ONLY.