Recombinant Human Tumor Necrosis Factor α/TNFα (C008)

目录 规格 价格
【TNFa/TNFSF2产品说明】:
Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed.
【TNFa/TNFSF2质量控制】:
Measured by the cytolysis of murine L929 cells in the presence of Actinomycin D.
ED50 is less than 0.03 ng/ml. Specific Activity of 3.0 x 10^7 IU/mg.
Greater than 95% as determined by reducing SDS-PAGE.
【TNFa/TNFSF2制剂】:
Lyophilized
【TNFa/TNFSF2保存】:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
【TNFa/TNFSF2复溶】:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
【关于TNFa/TNFSF2】:
TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease.
参考文献

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    Chronic inflammation aggravates metabolic disorders of hepatic fatty acids in high-fat diet-induced obese mice.
    Scientific reports(2015)

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    Galactose protects hepatocytes against TNF-α-induced apoptosis by promoting activation of the NF-κB signaling pathway in acute liver failure.
    Laboratory Investigation(2015)

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    Enhanced SCAP Glycosylation by Inflammation Induces Macrophage Foam Cell Formation.
    PloS one(2013)

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    MicroRNA-101 overexpression by IL-6 and TNF-α Inhibits cholesterol efflux by suppressing ATP-binding cassette transporter A1 expression.
    Experimental Cell Research(2015)

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    DDIT3 overexpression increases odontoblastic potential of human dental pulp cells.
    Cell proliferation(2014)

  • Yu-Sheng Chen Hong-Ru Li Ming Lin Gang Chen Bao-Song Xie Neng-Luan Xu Li-Fang Lin .
    Livin abrogates apoptosis of SPC-A1 cell by regulating JNKI signaling pathway..
    Mol Biol Rep (2010)

  • Lv Q, Zhen Q, Liu L, et al.
    AMP-kinase pathway is involved in tumor necrosis factor alpha-induced lipid accumulation in human hepatoma cells.
    Life sciences(2015)
For research use ONLY.