Protein A预装柱 (R001)

目录 规格 价格
prepacked column for protein purification

Protein A预装柱是预先用耦联金黄色葡萄球菌ProteinA的琼脂糖凝胶填好的柱子,采用开放式的设计,配备统一尺寸接口,可以与针筒注射器、蠕动泵、快速蛋白液相色谱(FPLC)及AKTA等纯化系统配套使用,通过一步纯化即可获得高纯度的IgG。

应用:

IgG和Fc片段的纯化,与Protein A的结合特性互补。

产品特点:
  • 操作简易:柱体已装好层析介质,可直接用于蛋白纯化
  • 结合能力强:广谱的IgG的结合能力可达50mg IgG/ml介质
  • 特异性好:去除了白蛋白和细胞表面结合域,减少了非特异性结合
技术实例:
Protein A预装柱
Novoprotein Protein A预装柱结合能力。
Lane 1: Loading Sample
Lane 2: Flow-through
Lane 3: Elution(reduced)
Lane 4: Protein Marker(PM001)
Lane 5: Elute(non-reducing)
参考文献

  • Liu D, Chen Y, Ding F, et al..
    Simultaneous production of butanol and acetoin by metabolically engineered< i> Clostridium acetobutylicum[J]..
    Metabolic engineering, 2015, 27: 107-114.(2015)

  • Ma M Z, Chu B F, Zhang Y, et al..
    Long non-coding RNA CCAT1 promotes gallbladder cancer development via negative modulation of miRNA-218-5p.
    Cell death & disease,2015, 6(1): e1583.(2015)

  • Xia Y, Niu Y, Cui J.
    The helicase activity of hyperthermophilic archaeal MCM is enhanced at high temperatures by lysine methylation.
    Frontiers in Microbiology(2015)

  • Yan-Fei Chen, Hongjun Chao, Ning-Yi Zhou.
    The catabolism of 2, 4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866. .
    Applied Microbiology and Biotechnology(2013)

  • Li F C, Gasser R B, Lok J B.
    Molecular characterization of the Haemonchus contortus phosphoinositide-dependent protein kinase-1 gene (Hc-pdk-1).
    Parasites & vectors(2016)

  • Chen W, Yu H, Ye L.
    Comparative Study on Different Expression Hosts for Alkaline Phytase Engineered in Escherichia coli.
    Applied biochemistry and biotechnology(2016)

  • Zhang W, Chen S, Liao Y, et al..
    Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa .
    Protein expression and purification(2013)
For research use ONLY.