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Quick List | |
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Price and timeline Service Terms Confidentiality Delivery Request to the Customers Why SinoBio Why Outsourcing How to order Frequently Asked Questions | |
Introduction
Almost any sequence under your demand, native or artificial,
will be available using gene synthesis method. DNA fragment up to 8,000 base
pairs can be synthesized in a very rapid turnaround time.
Additional components of the service include PCR, ligation,
cloning, and double-stranded confirmation sequenced. When coupled with SinoBio's
protein expression and purification service, the artificial genes can be
inserted into any site of the expression vectors the customers provided or
offered by SinoBio freely.
A lyophilized plasmid, e.coli glycerol stock as well as a
full report including the sequencing results and construction map will be
delivered to the customer.
Price and timeline
| Size of the gene | Term | Price(US$) |
| <150bp | 10 working days | 150/gene |
| 150~2000bp | 10~15 working days | 1.00/bp |
| 2~4 kb | 15~20 working days | 1.00/bp |
| above 4 kb | inquire | inquire |
Service Terms
The
sequence should be confirmed by the customer by email, designed by the clients
or SinoBio.
The synthesized gene will be cloned into the
standard vector you desired. Subclone into one of the expression vector will be
free if the synthesis service is coupled with SinoBio protein expression
service.
The typical DNA synthesis service include:
1. Design of synthesized DNA sequence
2. Synthesize target DNA.
3. DNA
sequencing.
4. Point mutation to
correct the mutation.
5. Lyophilize target plasmid
containing the synthesized gene.
6. Synthesis report.
Confidentiality
Your confidential sequence to us by
mail will be covered. We will sign non-disclosure or confidentiality agreements
when necessary.
A general template for MTA and CFA are
available and you may change the terms according to your requirements and
discuss it with SinoBio.
Delivery
Materials delivered to customers include the following:
.Lyophilized plasmid DNA containing the synthesized gene.
. e.coli stocks in glycerol contains the plasmid.
. Final DNA sequencing report and plasmid map;
. Gene synthesis report.
Shipment time:
The
typical delivery time for shipment is about 4 business days. This may vary
depending on where the customer located and accidentally, delayed by custom
check.
The final products will be shipped via fedx, DHL is
optional according to the customer's requirements.
Request to the Customers
1. Tell us the synthesized gene complete DNA sequences or ID number in NCBI
databank through the E-mail.
2. Provide us the plasmid (>
1ug) if you want us to insert the gene into your desired vector. US$300 (1~3kb)
or US$ 400 (>3kb) will be charged for one clone. In such a case, the following
information is also required:
Concentration and amount of the plasmid you provided
Map of the vector (whole sequence will be better)
Resistance marker
Sequencing
primer and primers for PCR identification (esp. when the full sequence of the
vector is not available.)
4. The customers need to pay for
50% fees as subscription.
5. For more genes to synthesize
or complex sequences, please contact us for a quote.
Correlative Service
Why SinoBio
Aims to
Expression: As a professional recombinant protein expression and
purification service provide, we are more experienced in design the sequence to
facilitate its expression in different host. And downstream gene cloning and
protein expression services immediately available;
Expertise: We hold over 3 years of expertise in the field of gene
synthesizing by using proprietary technology to synthesize the entire gene.
SinoBio can synthesize up to 10kb long genes, genes with repetitive sequences,
and genes with high percentage of G+C content.
Quality: Confirmed 100% match with your requested sequences by
double-strained DNA sequencing
Speed: Fast
turnaround time (1kb/10 days)
Price: Competitive prices as low as $1.00/bp
Confidentiality: Strictly confidential customer's data. Non-disclosure or
confidentiality agreements will be signed when needed
Why You need Synthesis
1. Not all of the cDNAs are available.
Nowadays, many genes are physically available from commercial suppliers.
However, there are a significant number of genes that exist only as digital
sequences. Also, Biological and Pharmaceutical research require genes of
man-made and predicted sequences that do not exist naturally. Custom gene design
and assembly provide a unique approach to obtain these otherwise unavailable
genes.
2. Full length gene synthesis can save money as
well as save your time. Weeks of time may be spent for cloning a full-length
cDNA, and another couple of weeks for sequencing validation, and mutagenesis are
needed sometimes. Just thinking about the salary you have to pay for a single
gene, no matter the cost for oligo synthesis, sequencing and reagents you spent.
3) Adjust the DNA sequence to facilitate gene expression in different hosts. The
adjust can be at least the following (but not limit to):
. Codon optimization
. RNA
secondary structure optimization
.
Restriction enzyme sites inclusion
. Addition of N- or C-terminal affinity tag
. Integration of upstream ribosome binding sites or a Kozak consensus sequences
for optimal expression
. Introns
removal
. Addition of elimination
of protein modification sites such as phosphorylation, glycosylation or
Pegylation sites
. Division of
genes into modules or cassettes for protein domain swapping, insertion, deletion
or duplication
. Introduction of
specific mutations within a particular gene module
. Chimeric/fusion protein production
Gene synthesis as the first choice:
. I know
about the protein sequence but no DNA sequence information available.
. The gene is from a rare species or expressed in a rare tissue or special
induction condition or it's a rare splicing variant and not available for
commercial supplier.
. I need changing the sequence in
complex way such as codon optimization, domain swapping, insertion or
duplication, multi position mutation.
. I care more about
the expression level.
Gene synthesis as the second choice:
We
don't recommend gene synthesis for all of your usage. The following are
suggested put gene synthesis as the second choice:
. I
have the gene on hand and/or the budget is limited.
. The
gene is available and there's credible reference for use of the gene in the same
purpose.
. The gene is available and the sequence is
predicted to match the host I intended.
. I want to study
the function of the native DNA sequence.
How to order
Please download the order form to specify your information, billing
information, and project information;
Please email the
completed order form to info@sinobio.net.
Please follow these instructions closely to speed
up your ordering process.
1. SinoBio Biotech adopt a non-disclosure policy to all
of our customers. To protect your proprietary information please mail, email
(legal@sinobio.net) or fax (8621-5079-8029) your confidentiality agreement to us
for sign. If intended, this should be done before any information is released to
us. If you don't have your own NDA/confidentiality agreement, you can find ours
at here. Feel free to modify it to suit your specific needs.
2. Call us (8621-5079-8028) or email us @, genesynthesis@sinobio.net, your
gene/protein sequence and particular manipulations you wish us to perform.
Particularly give clear instructions on the following options: codon
optimization vs. no optimization; host organisms; single host vs. multiple host
optimization; restriction sites or flanking sequences need to be incorporated
into both ends; restriction sites or flanking sequences need to be eliminated or
avoided internal to the gene; codon usage table preferred and any other specific
requirements.
3. Ask your purchasing department to fax
us (8621-5079-8029) the valid purchase order number. Work only starts with an
authorized purchase order number.
Frequently Asked Questions:
custom gene synthesis
1. Why use gene synthesis instead of PCR cloning?
2. Does codon optimization really matter?
3. When I can use the native sequence for gene expression
4. What is the standard vector for SinoBio gene synthesis?
5. Can SinoBio subclone the gene into the vector of my choice?
6. How can I place my order?
7.
What is the time frame for delivery ?
8.
How much does it cost to ship outside the China?
9.
What kinds of payments do you accept?
10.
Do you provide protein expression services for codon-optimized genes?
11. Is optimization needed when the host I choused to expression
the protein is same to the gene source?
12.
Should I include the signal peptide?
13.
Should I include the Transmembrane domain?
14.
Should I include a tag?
15. When I
should express a gutless protein, without any additional amino acid?
16. When should I required the native structure?
17.
Is soluble expressed protein from e.coli conserve its biological
activity?
________________________________________
1). Why use gene synthesis instead of PCR cloning?
A:
PCR cloning, the most common method of obtaining genes, may not be able to
produce a gene of sufficient quality or even at all. First, a cDNA library for a
specific type of tissue has to be prepared or purchased, which requires either
time or expense. Second, the gene must be abundant in that cDNA library or it
will be very difficult to clone any useful way. Third, when PCR is successful,
its products may have mutations or single nucleotide polymorphisms (SNP), which
can cause problems during experimentation or other applications. SinoBio gene
synthesis guarantees delivery of workable quantities of precise, unmutated genes
at a far more economical price than PCR cloning.
2).
Does codon optimization really matter?
A: It matters as much as protein
expression matters. Different organisms have different codon usage preferences.
For example, the preferred codons used in a human gene may be rare in bacteria.
This can cause problems when researchers attempt to express human genes in E.
coli. Using our optimized synthetic genes, many of our customers have reported
dramatic increases in protein expression.
3).
When I can use the native sequence for gene expression?
A: Maybe only one
third of mammalian genes can be expressed directly in e.coli. Kindly optimized
DNA sequence can be expressed better. But if your budget is limited and you have
the cDNA on hand, or there's reliable reference to express the cDNA in the host
you targeted, you can use the native sequence for your gene expression.
4). What is the standard vector for SinoBio gene synthesis?
A:
pSBlunt is the standard vector of SinoBio Corporation. In general, synthetic
genes will be cloned into Sma I or EcoR V sites of the standard vector.
5). Can SinoBio subclone the gene into the vector of my
choice?
A:
SinoBio can subclone the synthetic gene into the vector of your choice for a
reasonable additional fee. The vector must be provided by the customer and must
be a commercial vector with a full-length sequence.
When DNA synthesis is coupled with SinoBio's protein expression and purification
service, subcloning into one of the expression vector is free.
6). How can I place my order?
A: You can give SinoBio
your sequence and payment information via email or fax.
7). What is the time frame for delivery ?
A: For genes
up to 1 kb, the delivery time is 12 business days. One week is added for each
additional 1 kb. For subcloning into a vector provided by customer, an extra 1-2
weeks are needed.
8). How much does it cost to
ship?
A: We ship our products to many countries outside the China. The
shipping charge is generally $35 per shipment.
9).
What kinds of payments do you accept?
A:
SinoBio can only accepts checks and wire transfer.
10).
Do you provide protein expression services for codon-optimized genes?
A:
SinoBio also provides protein expression and purification services for the
synthesized genes. SinoBio can express protein in a variety of systems including
bacteria, yeast, baculovirus, and mammalian cells.
11). Is optimization needed when the host I chosen to
expression the protein is same to the gene source?
A: Codon usage is one kind of gene expression regulation. The same organizm
may use rare codon to expression it gene. In such a case you'd better chose
codon optimization.
12). Should I include the signal peptide?
A: In most case you should ommit the signal peptide when you try to express
your gene in e.coli. The yeast and insect cells use different signal peptide in
most case. Even in different mammalian cells the capability of secretion is
different. Try to change the peptide to more fammiliar one such as IgK signal
peptide instead of the original peptide when it's really need to be included.
13). Should I include the Transmembrane domain?
A: Transmembrane domain is difficult to be purified. Try to express partial
of your protein to ommit the TM domain if it's not your purpose to study the TM
domain.
14). Should I include a tag?
A: Fusion to a tag can facilitate your protein expression and purification.
It's can be digested if needed. In lots of case include a tag will not affect
your study so it needn't to be removed.
15). When I should express a gutless protein, without
any additional amino acid?
A: Lots of studies use tagged protein or contains several additional aa on
the protein. If you want to get the protein as biological drug, express a
gutless protein or remove the tag with protease is recommend.
16). When should I required the native structure?
A: In case of functional study, drug development, structure study, the
native structure is required. We strongly recommend to express the protein in
mammalian cells, insect cells or soluble expression in e.coli. Proteins
expressed in e.coli in inclusion body can also be refolded properly.
17). Is soluble expressed protein from e.coli conserve
its biological activity?
A: Generally, soluble expressed protein in e.coli will conserve the native
structure and it's biological activities. but we do find some proteins soluble
expressed in e.coli cannot show it's predicted biological activity.
