Pfu 2X Master Mix
(Cat. No.: E006)
Description:
Pfu 2X Master Mix is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. The Pfu 2X Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2¨C3kb.
PCR Master Mix, 2X:
50 units/ml of Pfu DNA Polymerase supplied in a proprietary reaction buffer (pH 8.5), 400µM each of dATP, dGTP, dCTP, dTTP,
Features:
Fast: Set up reactions in less than a minute.
Sensitive: Amplify as little as 2 copies of target template.
High Fidelity: Pfu 2X Master Mix exhibits the lowest error rate of any thermostable DNA polymerase.
Convenient: One tube, one pipetting step.
Complete: Reagents, including Pfu DNA Polymerase, MgSO4, dNTPs and buffers, in one tube.
Scalable: Set up 10µl, 25µl or 50µl reactions.
Stable: Stable for hundreds freeze-thaw cycles.
Performance Guarantee: SinoBio PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any SinoBio PCR product, we will send a replacement or refund your account.
Applications:
Pfu 2X Master Mix is recommended for use in PCR, primer extension reactions and other applications that demand high fidelity.
Unit Definition:
One unit Pfu DNA Polymerase is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into acid-insoluble material in 30 minutes at 75oC.
Quality Control Tests:
PCR, activity, endo- and exo- nuclease.
Storage:
Store at -20oC.
