SinoBio Biotech's purification development group has developed purification processes for a wide variety of recombinant proteins. Many of these process development projects are undertaken in collaboration with the customer's process development and analytical scientists. A wide range of chromatographic operations have been used for the purification of recombinant proteins (e.g. ion exchange, hydrophobic interaction, affinity, ceramic Hydroxyapatite, metal chelate, gel permeation, etc.).
Scientists in SinoBio Biotech have taken part in 9 protein drugs process development. That is one third of biologics in China protein drug market. In the past three years SinoBio Biotech have established about 400 different proteins manufacture process scale from milligram to tens of gram. About 40 cytokines and tens of enzymes are qualified by well known reagent and cytokine sellers and contract all over the world. Five of them are get into preclinical trials and one of them filed IND.
Points to consider in developing a biopharmaceutical process include: protein purity, scale-up requirements, yield, practical manufacturability and overall cost.
A typical process development protocol for recombinant protein includes:
| 1. | Optimization of Expression Systems |
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| 2. | Optimization of fermentation Conditions |
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| 3. | Development of a purification SOP |
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| 4. | Confirmed by the customer and technique transfer |
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PRODUCT DEVELOPMENT:
SinoBio Biotech provide flexible services to meet your diverse requirements. Product development service coupled with SinoBio's contract protein manufacture service can provide you materials you needed with minimal cost esp. when customer just need the protein as raw material and don't want set up their own manufacture facilities.
SinoBio Biotech provide serials quality controls such as SDS-PAGE, HPLC, MS, MS/MS, Amino acid sequencing, endotoxin control to ensure the quality of the final products meet your requirements.
A typical product development procedure includes:
| 1. | Discussion with the customers requirements |
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| 2. | Development the products by SinoBio's scientists |
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| 3. | Confirmed by the customer and shipment. |
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| 4. | The customer will get. |
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Please contact us for details about process development and product development by email to info@sinobio.net or call us 8621-5079-8029.
Service Terms
SinoBio Biotech provide recombinant protein services include:
Expression with diverse expression
systems
such as
e.coli,
yeast,
insect cells and
mammalian cells.
Manufacture of target
protein( Product development)
OR
establish manufacture
procedure for the customer (Process development).
Protein Refolding and Protein
modification such as PEGylation
Protein Analysis
such as
AA sequencing,
MS,
HPLC,
endotoxin test,
biological activity assay...
Confidentiality
Your
confidential sequence to us by mail will be covered. We will sign non-disclosure
or confidentiality agreements when necessary.
A general
template for MTA and CFA are available and you may change the terms according to
your requirements and discuss it with SinoBio.
Delivery
Materials
delivered to customers include the following:
1.
Expression e.coli strain in glycerol.
2. DNA sequence data
(When necessary.).
3. Required final products in
conventional buffer.
4. Manufacture reports, including the
major process, interim results, quality control.
Delivery time:
The typical delivery time for shipment
is about 4 business days. This may vary depending on where the customer located
and accidentally, delayed by custom check.
The final
products will be shipped via fedex. DHL is optional according to the customer's
requirements.
Request to the Customers
1. Tell us your purpose such as products required or process required.
2. Provide us the
requirements of the final products essentially the purity and amount, other
quality control requirements are optional with additional charge such as HPLC,
AA Sequencing, MS, MS/MS, biological activity, endotoxin control etc. For
details please read protein analysis.
3. A purification service can begin from electronic sequence
of your target protein or partially purified products.
4. The customers need to pay for 50% fees as subscription for the first order.
5. For more genes to be expressed or complex project, please contact us for a
quote.
Correlative Service
Gene Synthesis
Polyclonal Antibody Production
Protein Analysis (HPLC, LAL, AA Sequencing, MS, MS/MS)
Protein PEGylation
Protein Refolding
Activity assay
for some of the proteins
Expression in
P.Pichia
Expression in
Insect Cells
Expression in
Mammalian Cells
Expression in
e.coli
Why SinoBio
Professional:
SinoBio Biotech focus on recombinant protein manufacture only, and the focus
makes us more professional. All of the group leaders are come from big
biopharmaceuticals with more than 10 years experience on protein production. The
company are well equipped with Bioengenering fermentor, AKATA, HPLC and we can
provide cGMP standard for your production. The fermentation scale is from liters
to 500 liters. Final production is scale from milligram to tens of gram.
Expertise: SinoBio's protein experts have more
than 300 different protein purification experience, involved in NINE biologics
development, most of them are in market and some of them in clinical trails.
They also developed about 40 qualified cytokines and tens of biological enzymes.
Quality: Diverse quality control method available for your choice such as
amino acid sequencing, MS, MS/MS, LAL test, HPLC in addition to conventional SDS-PAGE
analysis.
Speed: Fast turnaround time (~10 days for
a basic order)
Price: Competitive prices as low as 800$
Confidentiality: Strictly confidential customer's data. SinoBio will sign
non-disclosure or confidentiality agreements when needed.
How to
order
Please download the order form to specify your information, billing
information, and project information;
Please email the
completed order form to info@sinobio.net
Please follow these instructions closely to speed up
your ordering process.
1. SinoBio Biotech adopt a non-disclosure policy to all
of our customers. To protect your proprietary information please mail, email
(info@sinobio.net) or fax (+8621-5079-8029) your confidentiality agreement to us
for sign. If intended, this should be done before any information is released to
us. If you don't have your own NDA/confidentiality agreement, you can find ours
at here. Feel free to modify it to suit your specific needs.
2. Call us (+8621-5079-8029) or email us info@sinobio.net, your gene/protein
sequence and particular manipulations you wish us to perform. Particularly give
clear instructions on the following options: codon optimization vs. no
optimization; host organisms; single host vs. multiple host optimization. Total
amount of final protein you required, the qualities and QC terms you required.
