Service Terms
   The sequence should be confirmed by the customer by email, designed by the clients or SinoBio.
    The synthesized gene will be cloned into the standard vector you desired. Subclone into one of the expression vector will be free if the synthesis service is coupled with SinoBio protein expression service.
 

ANALYSIS METHOD	N-Terminal Protein Sequencing
Description
	Automated protein sequencing has evolved considerably with greater sensitivity, speed and ease of operation. Advances in mass spectrometry have now taken the center stage for protein identification. MS provides high throughput automation with more precise and powerful protein analysis. However, N-terminal sequencing by Edman degradation still continues to complement MS in difficult protein identifications.
REQUIREMENTS TO THE CUSTOMERS
	10 pmol or more of sample is required for routine analysis samples can be submitted in lyophilized/liquid form in Eppendorf tube or on a PVDF membrane
RESULTS PROVIDED TO THE CUSTOMERS
	Turn around times: 1-3 working days. No minimum number of residues required. Prices include desalting and adsorptive binding of the sample to PVDF membrane.     The customers will get a report contains the analyzed sequence, sequencing map and a general description of analysis method.

ANALYSIS METHOD	MS/MS/MS
Description
	SinoBio Biotech offers molecular weight determination of proteins and peptides by Mass Spectrometry Method. Analysis is performed by Matrix Assisted Laser Desorption Ionization (MALDI) or by Electrospray Ionization technique.
REQUIREMENTS TO THE CUSTOMERS
	10 pmol of protein/peptide or more is required in solution or lyophilized Protein in solution: as concentrated as possible; total volume 10-20 ml The higher the expected protein mass, the more protein is needed to obtain good mass data (10 pmol for 20 kD protein)
RESULTS PROVIDED TO THE CUSTOMERS
	Turn around times: 5 working days.  Prices include desalting and adsorptive binding of the sample to PVDF membrane.     The customer will get a report with the following contents:     1. MS or MS/MS map.     2. A general illuminant of the analysis results.     3. General description of analysis method.

ANALYSIS METHOD	HPLC analysis
Description
	SinoBio Biotech offers various chromatographic methods to analyze your protein purities. You may chose RP-HPLC, SEC-HPLC or IEX HPLC for your own sample analysis or as the quality requirements to SinoBio Biotech's contract protein services.
REQUIREMENTS TO THE CUSTOMERS
	At least 50ul sample with a concentration that more than 0.1ug/ul are required for HPLC analysis. Lyophilized samples are also acceptable with total amount of more than 5ug.    The formulation of your samples should not contains height concentration of salts, strong denaturing reagent such as urea.    For details of sample requirements please contact our scientist by email: info@sinobio.net
RESULTS PROVIDED TO THE CUSTOMERS
	Turn around times: 1-3 working days. Sometimes we may need more time to setup a individual analysis process to meet the special requirement.     The customer will received an analysis report include the analysis method, final result and a general analysis of the results.

ANALYSIS METHOD	ELISA
Description
	SinoBio Biotech offers ELISA analysis for protein identification, protein concentration determinant or antibody titers. xxxx can analysis reactions at the following wave length:     280nm, 310nm..... .
REQUIREMENTS TO THE CUSTOMERS
	Enough samples for analysis, determined by the requirements of your experiments.     Antibodies.     manu of your antibodies.     Labeling information of your antibodies.
RESULTS PROVIDED TO THE CUSTOMERS
	The final analysis reports will contains a standard curves, raw data of ELISA analysis, general conclusion and analysis of the data.

Confidentiality
    Your confidential sequence to us by mail will be covered. We will sign non-disclosure or confidentiality agreements when necessary.
    A general template for MTA and CFA are available and you may change the terms according to your requirements and discuss it with SinoBio.

Delivery
    Materials delivered to customers include the following:
    1. Expression e.coli strain in glycerol.
    2. DNA sequence data (When necessary.).
    3. Required final products in conventional buffer.
    4. Manufacture reports, including the major process, interim results, quality control.
    Delivery time:
    The typical delivery time for shipment is about 4 business days. This may vary depending on where the customer located and accidentally, delayed by custom check.
    The final products will be shipped via fedex. DHL is optional according to the customer£¦#8217;s requirements.

Request to the Customers

    1. Tell us the target DNA sequences or Genebank accession number or protein sequence through the E-mail.
    2. Provide us the requirements of the final products essentially the purity and amount, other quality control requirements are optional with additional charge such as HPLC, AA Sequencing, MS, MS/MS, biological activity, endotoxin control etc. For details please read protein analysis.
    4. The customers need to pay for 50% fees as subscription for the first order.
    5. For more genes to be expressed or complex project, please contact us for a quote.

Correlative Service
    Gene Synthesis
    Polyclonal Antibody Production
    Protein PEGylation
    Protein Refolding
    Activity assay for some of the proteins
    Expression in P.Pichia
    Expression in Insect Cells
    Expression in Mammalian Cells
    Expression in e.coli


Why SinoBio
    Professional: SinoBio Biotech focus on recombinant protein manufacture only, and the focus makes us more professional. All of the group leaders are come from big biopharmaceuticals with more than 10 years experience on protein production. The company are well equipped with Bioengenering fermentor, AKATA, HPLC and we can provide cGMP standard for your production. The fermentation scale is from liters to 500 liters. Final production is scale from milligram to tens of gram.
    Expertise: SinoBio's protein experts have more than 300 different protein purification experience, involved in NINE biologics development, most of them are in market and some of them in clinical trails. They also developed about 40 qualified cytokines and tens of biological enzymes.
    Quality: Diverse quality control method available for your choice such as amino acid sequencing, MS, MS/MS, LAL test, HPLC in addition to conventional SDS-PAGE analysis.
    Speed: Fast turnaround time (~10 days for a basic order)
    Price: Competitive prices as low as 800$
    Confidentiality: Strictly confidential customer's data. SinoBio will sign non-disclosure or confidentiality agreements when needed.




How to order
    Please download the order form to specify your information, billing information, and project information;
    Please email the completed order form to info@sinobio.net.

    Please follow these instructions closely to speed up your ordering process.

    1. SinoBio Biotech adopt a non-disclosure policy to all of our customers. To protect your proprietary information please mail, email (legal@sinobio.net) or fax (8621-5079-8029) your confidentiality agreement to us for sign. If intended, this should be done before any information is released to us. If you don't have your own NDA/confidentiality agreement, you can find ours at here. Feel free to modify it to suit your specific needs.

    2. Call us (8621-5079-8028) or email us @, genesynthesis@sinobio.net, your gene/protein sequence and particular manipulations you wish us to perform. Particularly give clear instructions on the following options: codon optimization vs. no optimization; host organisms; single host vs. multiple host optimization; restriction sites or flanking sequences need to be incorporated into both ends; restriction sites or flanking sequences need to be eliminated or avoided internal to the gene; codon usage table preferred and any other specific requirements.

    3. Ask your purchasing department to fax us (8621-5079-8029) the valid purchase order number. Work only starts with an authorized purchase order number.


Frequently Asked Questions: Protein Analysis
    1) May Previously stained gels be blotted?
    2) How to detect the transfer efficiency?
    3) Could the membranes reprobed?
    4) What is purity requirements for protein sequencing?
    5) How to prepare samples for protein sequencing?
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1) May Previously stained gels be blotted?
    Previously stained gels may also be blotted.


2) How to detect the transfer efficiency?
    a process that can be monitored by reversible staining or by Ponceau S staining.


3) Could the membranes reprobed?
    Membranes may be stripped and reprobed.. 


4) What is purity requirements for protein sequencing?
    The purity of the protein should be 85% or higher. Contaminating peptides or proteins interfere with the Edman chemistry and contribute to a higher noise level. The lower the amount of protein, the higher the purity is required. .


5) How to prepare samples for protein sequencing?
    When preparing samples for microsequencing, it is best to limit sample manipulations. When dealing with solution samples, never bring the sample to complete dryness. Based on extensive studies, we have found that SDS-PAGE or 2D IEF-SDS-PAGE (or optionally transferred to PVDF) is the best method for recovery of low quantities of protein. However, several other methods are listed below: DESALTING: the best method for desalting is SDS-PAGE . Samples can be desalted by reversed-phase HPLC, but the recoveries are variable. ELECTROELUTION: if proteins have been prepared by electroelution, they should be precipitated (TCA, ethanol) following electroelution in order to remove detergents and salts. Alternatively reversed-phase HPLC can be used but the recovery is variable. Proteins submitted for internal sequence analysis are routinely reduced and carboxyamidomethylated prior to digestion. Proteins transferred to PVDF: Samples can be separated by 1D (Laemmli SDS-PAGE) or 2D (O'Farrell) gels followed by electrophoretic transfer to PVDF. Enough protein should be loaded in a lane so that each band or spot contains 1-5 µg of sample. Lower amounts can be pooled for analysis after blotting. Artificial blockage of the N-terminus during sample preparation or electrophoresis has been reported to be a factor in reducing the amount of material that can be sequenced following electrophoresis (14). However, if fresh reagents of the highest quality are used, it should not present a problem as determined by the center. PVDF membranes should have a high protein binding capacity (0.1-0.22 µm pore size) so as to retain the most protein. Suggested suppliers are ProBlott (Applied Biosystems), Westran (BioRad), Novex, and Immobilon Psq (Millipore). Immobilon P (Millipore) retains less protein but can still be used. Even under the best circumstances there is always sample loss during the transfer step: therefore, if very little material is available, the sample should be submitted as a gel band to minimize sample loss..