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Protein Purification
    Scientists in SinoBio Biotech have taken part in 9 protein drugs process development. That is one third of biologics in China protein drug market. In the past three years SinoBio Biotech have established about 400 different proteins manufacture process scale from milligram to tens of gram. About 40 cytokines and tens of enzymes are qualified by well known reagent and cytokine sellers and contract all over the world. Five of them are get into preclinical trials and one of them filed IND.

    SinoBio Biotech provide a systemic protocol for protein purification to ensure your project be finished in a timely and reliable manner. The whole   include:

    A well documented flowchart direct scientist in SinoBio Biotech what should do in the next step.
    Expression screen include screen of different medium, addictives and culture condition/expression condition to improve the expression level/conserve the protein activities. e.g., different sodium and amino acid, or detergent sometimes will be added to boost your target protein expression. Different temperature, induction reagent concentration will also be optimized. Carbon and nitrogen source will be adjusted according to different strains. For mammalian cell culture, we can screen for optimized growth factors and medium composition improve the expression level.
    Target protein localization will be tested for e.coli expression to determine if they expressed in inclusion bodies or soluble expressed, expression conditions will be also optimized to improve the proteins in desired patron.
    Different methods can be used to disrupt the cells: Ultrasonic, ...
    SinoBio's purification scientist will chose some of the resins according to the properties of your target protein to purify the proteins. The resins include:
        IEX, such as DEAE, QAE, SP, CM,
        HIC, such as phenyl- octyl-£¦#8230;£¦#8230;
        SEC, such as
        Affinity according to the affinity tag or properties of your target proteins such as GST, NTA, BLUE, HEPERIN, AVIDIN, FLAG TAG£¦#8230;£¦#8230;

    Fusion tags can be removed according to your requirement. Different protease can be chose such as Enterokinase, Thrombin, Factor Xa, TEV protease, SUMO protease...
    SinoBio only provide soluble protein in conventional buffer to our customers. This means the products we provided will not contain any denature reagent such as urea.
    If the protein expressed in inclusion body, the protein will be refolded with a common method provided by Sinobio to ensure the solubility of your protein. In this case, we will not promise the biological activities. But lots of our customers still found the proteins are correctly refolded and active in there assay.
    If the customer requires the activities of the target protein, a systemic refolding process can be required, by which, a serials of refolding condition will be screened such as pH, oxidant, detergents, and other addictives. An optimized refolding condition can be acquired after a full refolding screen.
    SDS-PAGE purity will be tracked in all the process. Other quality analysis can be choose such as HPLC, MS, MS/MS, AA sequencing. For details please read PROTEIN ANALYSIS.

Service Terms
    SinoBio Biotech provide recombinant protein services include:
        Expression with diverse expression systems
            such as e.coli, yeast, insect cells and mammalian cells.
        Manufacture of target protein( Product development)
            OR establish manufacture procedure for the customer (Process development).
        Protein Refolding and Protein modification such as PEGylation
        Protein Analysis
            such as AA sequencing, MS, HPLC, endotoxin test, biological activity assay...

Confidentiality
    Your confidential sequence to us by mail will be covered. We will sign non-disclosure or confidentiality agreements when necessary.
    A general template for MTA and CFA are available and you may change the terms according to your requirements and discuss it with SinoBio.

Delivery
    Materials delivered to customers include the following:
    1. Expression e.coli strain in glycerol.
    2. DNA sequence data (When necessary.).
    3. Required final products in conventional buffer.
    4. Manufacture reports, including the major process, interim results, quality control.
    Delivery time:
    The typical delivery time for shipment is about 4 business days. This may vary depending on where the customer located and accidentally, delayed by custom check.
    The final products will be shipped via fedex. DHL is optional according to the customer's requirements.

Request to the Customers

    1. Tell us your purpose such as products required or process required.
    2. Provide us the requirements of the final products essentially the purity and amount, other quality control requirements are optional with additional charge such as HPLC, AA Sequencing, MS, MS/MS, biological activity, endotoxin control etc. For details please read protein analysis.
    3. A purification service can begin from electronic sequence of your target protein or partially purified products.
    4. The customers need to pay for 50% fees as subscription for the first order.
    5. For more genes to be expressed or complex project, please contact us for a quote.

Correlative Service
    Gene Synthesis
    Polyclonal Antibody Production
    Protein Analysis (HPLC, LAL, AA Sequencing, MS, MS/MS)
    Protein PEGylation
    Protein Refolding
    Activity assay for some of the proteins
    Expression in P.Pichia
    Expression in Insect Cells
    Expression in Mammalian Cells
    Expression in e.coli

Why SinoBio
    Professional: SinoBio Biotech focus on recombinant protein manufacture only, and the focus makes us more professional. All of the group leaders are come from big biopharmaceuticals with more than 10 years experience on protein production. The company are well equipped with Bioengenering fermentor, AKATA, HPLC and we can provide cGMP standard for your production. The fermentation scale is from liters to 500 liters. Final production is scale from milligram to tens of gram.
    Expertise: SinoBio's protein experts have more than 300 different protein purification experience, involved in NINE biologics development, most of them are in market and some of them in clinical trails. They also developed about 40 qualified cytokines and tens of biological enzymes.
    Quality: Diverse quality control method available for your choice such as amino acid sequencing, MS, MS/MS, LAL test, HPLC in addition to conventional SDS-PAGE analysis.
    Speed: Fast turnaround time (~10 days for a basic order)
    Price: Competitive prices as low as 800$
    Confidentiality: Strictly confidential customer's data. SinoBio will sign non-disclosure or confidentiality agreements when needed.


How to order
    Please download the order form to specify your information, billing information, and project information;
    Please email the completed order form to info@sinobio.net

    Please follow these instructions closely to speed up your ordering process.

    1. SinoBio Biotech adopt a non-disclosure policy to all of our customers. To protect your proprietary information please mail, email (info@sinobio.net) or fax (+8621-5079-8029) your confidentiality agreement to us for sign. If intended, this should be done before any information is released to us. If you don't have your own NDA/confidentiality agreement, you can find ours at here. Feel free to modify it to suit your specific needs.

    2. Call us (+8621-5079-8029) or email us info@sinobio.net, your gene/protein sequence and particular manipulations you wish us to perform. Particularly give clear instructions on the following options: codon optimization vs. no optimization; host organisms; single host vs. multiple host optimization. Total amount of final protein you required, the qualities and QC terms you required.