Taq Plus DNA Polymerase

(Cat. No.: E009)

Description:

Taq Plus DNA Polymerase is a special formulation designed for amplify large fragment. The main component is Taq DNA Polymerase, and some special addictives such as Pfu DNA Polymerase are added to enhance the efficiency of amplification reaction. Theoretically, Taq Plus produces significantly higher yields of PCR products than ordinary Taq Polymerase, especially for fragments >1 kb, and can amplify up to 30 kb. Taq Plus also contains a proofreading activity that reduces the error rate of Taq Polymerase. Taq Plus is suitable as a direct replacement for ordinary Taq Polymerase in most applications.

Features:

High fidelity: with an error frequency of 1.6X10-6 during DNA synthesis.

Higher yield: Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb.

Rely on a Performance-Tested Enzyme: SinoBio PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account.

Applications:

Long PCR (up to 30 kb), PCR cloning, RT-PCR etc.

Unit Definition:

One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 74oC.

Quality Control Tests:

PCR, Activity, endonuclease/nickase, Specific performance test.

Storage:

Taq Plus DNA polymerase in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20 should be stored at -20oC.

10X Reaction Buffer with MgCl2:

500mM KCl, 100mM Tris-HCl (pH 9.0 at 25oC) and 1% Triton X-100£¬ 100mM (NH4)2SO4, and 35mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP.

Reaction Condition:

DNA synthesis is performed in 100µl of mixture containing 20-200µM dNTPs, 0.3-1µM Primers, 0.1-0.250mg of template DNA, 10 µl of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil.

Initially, denature the reaction by incubating at 95oC for 5 minutes and then cool to 40-68oC for 5 minutes to allow the primers to anneal to the template DNA.