Taq Plus 2X Master Mix
(Cat. No.: E007)
Description:
Taq Plus 2X Master Mix is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase, dNTPs, MgCl2 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. The Taq Plus 2X Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 1¨C10kb.
PCR Master Mix, 2X:
50 units/ml of Taq Plus DNA Polymerase supplied in a proprietary reaction buffer (pH 8.8), 400µM each of dATP, dGTP, dCTP, dTTP,
Features:
Fast: Set up reactions in less than a minute.
Sensitive: Amplify as little as 2 copies of target template.
High fidelity: with an error frequency of 1.6X10-6 during DNA synthesis.
Higher yield: Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb.
Convenient: One tube, one pipetting step.
Complete: Reagents, including Taq Plus DNA Polymerase, MgCl2, dNTPs and buffers, in one tube.
Scalable: Set up 10µl, 25µl or 50µl reactions.
Stable: Stable for hundreds freeze-thaw cycles.
Performance Guarantee: SinoBio PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any SinoBio PCR product, we will send a replacement or refund your account.
Applications:
Long PCR (up to 30 kb), PCR cloning, RT-PCR etc.
Unit Definition:
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 74oC.
Quality Control Tests:
PCR, Activity, endonuclease/nickase, Specific performance test.
Storage:
Store at -20oC.
