Recombinant Human Interleukin-8 (8-79) (IL8 /72)
(Cat. No.: C035)
Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states.
Description:
Recombinant Human IL-8 (8-79) produced in E. coli is a single, non-glycosylated polypeptide chain containing 72 amino acids and having a molecular mass of 8452 Dalton.
Quality Control:
Biological activity: rHuIL-8 /72 is fully biologically active when compared to standard. The ED50 as determined by its chemotaxis of hCXCR-2 transfected mouse BaF/3 cells is less then 2 ng/ml, corresponding to a Specific Activity of 5.0 x 105 IU/mg.
Purity: Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Molecular weight: 9 KD+/-10% determined by reduced SDS-PAGE.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Lys-Glu-Leu.
Endotoxin: Less than 0.1ng/µg (1 IEU/µg) determined by LAL test.
Formulation:
Lyophilized from a concentrated (1mg/ml) solution containing 20mM PBS pH-7.4 and 50mM sodium chloride.
Storage:
Lyophilized rHuIL-8 /72 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution rHuIL-8 /72 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Reconstitution:
It is recommended to reconstitute the lyophilized rHuIL-8 /72 in sterile 18M¦¸-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
