Recombinant Human Bone Morphogenetic Protein -2 (BMP2)
(Cat. No.: C012)
Background:
Belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Description:
Recombinant Human Bone Morphogenetic protein-2 produced in E. coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton.
Quality Control:
Biological activity: BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less then 50 ng/ml, corresponding to a specific activity of 2.0 x 104 IU/mg
Purity: Greater than 95% as determined by
(a) Analysis by SEC-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of Bone Morphogenetic protein-2.
Formulation: BMP2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.
Storage: Lyophilized BMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BMP2 should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Reconstitution: It is recommended to reconstitute the lyophilized rHuBMP2 in sterile 20mM Acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
