Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
(Cat. No.: C003)
Background:
GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
Description:
GM-CSF is a highly purified protein with a molecular weight of approximately 14kDa. It contains 123 amino acids residues. The protein is produced by recombinant DNA technology using a genetically engineered E. coli strain containing the human GM-CSF gene.
Quality Control:
Biological activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1 ng/ml, corresponding to a Specific Activity of 1.0 x 107 IU/ mg.
Purity: Greater than 95% as determined by
(a) Analysis by SEC-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Isoelectric Point: the main zone between 4.5~5.7 analysis by IEF.
Amino-Acid Sequence: The sequence of the first fifteen N-terminal amino acids was determined and was found to be (Met)-Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr-Gln-Pro-Trp-Glu-His.
Endotoxin: Less than 0.03ng/µg (0.03IEU/µg) determined by LAL test.
Formulation: The protein was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH=7.4+/-0.1.
Storage: Lyophilized rHuGM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution rHuGM-CSF should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Reconstitution: It is recommended to reconstitute the lyophilized rHuGM-CSF in sterile 18M¦¸-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
