• Home
• Service
  • Process
  • Product
  • Package
  • Additional Services
• Products
  • Cytokines
  • Enzymes +
  • Place Order
• About
• Contact
• Login

Recombinant Human Interleukin-1 Receptor Antagonist (IL-1RN)
(Cat. No.: C033)

Background:
        Interleukin-1 receptor antagonist (IL-1RN) is a member of the IL-1 family. Endogenous IL-1RN is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups.
        IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL-1RN.
        The regulated expression of IL-1RN in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1RN is markedly enhanced by IL-1, TNF-alpha, or PDGF.

Description:
        Recombinant Human IL-1RN produced in E. coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17258 Dalton.

Quality Control:
        Biological activity: The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 10⁶ IU/mg.
        Purity: Greater than 95% as determined by
                    (a) Analysis by RP-HPLC.
                    (b) Anion-exchange FPLC.
                    (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel..
        Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.
        Endotoxin: Less than 0.1ng/µg (1IEU/µg) of IL-1RN.

Formulation: IL-1RN was lyophilized after extensive dialysis against PBS.

Storage: Lyophilized rHuIL-1RN although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuIL-1RN should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
        Please avoid freeze-thaw cycles.

Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-1RN in sterile 18M¦¸-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.