Recombinant human Interleukin-15 (IL15)
(Cat. No.: C016)

Background:
        Interleukin-15 (IL-15) is a cytokine that possesses a variety of biological functions, including stimulation and maintenance of cellular immune responses. IL-15 stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R.

Description:
        Recombinant Human IL-15 produced in E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12774 Dalton.

Quality Control:
        Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 10⁶ IU/mg.
        Purity: Greater than 98.0% as determined by:
                    (a) Analysis by RP-HPLC.
                    (b) Anion-exchange FPLC.
                    (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
        Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Trp-Val-Asn-Val.
        Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuIL-15.

Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 5mM Tris pH 8.0.

Storage: Lyophilized rHuIL-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuIL-15 should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
        Please avoid freeze-thaw cycles.

Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-15 in sterile 18M¦¸-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.