Recombinant Human Interleukin-2 (IL2)
(Cat. No.: C013)
Background:
In vitro studies performed on human cell lines demonstrate the immunoregulatory properties of IL-2 including: 1) enhancement of lymphocyte mitogenesis and stimulation of long-term growth of human IL-2 dependent edll lines; 2) enhancement of lymphocyte cytotoxicity; 3) induction of killer cell (lymphokine-activated (LAK) and natural (NK)) activity; 4) dnduction of interferon-gamma production.
Description:
Recombinant Human interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the human IL-2 gene, and the resulting expression clone encodes a modified human IL-2. This recombinant form differs from native interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2.
Quality Control:
Biological activity: The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less then 0.1 ng/ml, corresponding to a Specific Activity of 1.0 x 107 IU/ mg.
Purity: Greater than 95% as determined by
(a) Analysis by SEC-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Isoelectric Point: the main zone between 6.5~7.5 analysis by IEF.
Amino-Acid Sequence: The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys-Thr-Gln-Leu- Gln-Leu-Glu.
Endotoxin: Less than 0.1ng/µg (0.1IEU/µg) determined by LAL test.
Formulation: The protein (1.1mg/ml) was lyophilized after extensive dialysis against 0.17mg sodium monobasic and 0.89mg dibasic sodium phosphate buffer to a pH=7.5.
Storage: Lyophilized rHuIL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution rHuIL-2 should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-2 in sterile 18M¦¸-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
